Reference set (classifier version: 8.0)

The reference set is highly reminiscent of the WHO classification of brain tumors but not identical. For the constriction of the reference set at least 8 typical cases of each histological subtype described in the WHO classification of brain tumors were collected and underwent critical histological review. If available, only cases at first diagnosis and prior to radiation or chemotherapy were selected. For the majority of cases DNA was extracted from formalin fixed paraffin embedded tumor tissue. A special focus was set on tumor purity with rigorous dissection of areas with maximal tumor cell content.

The cases were then defined as a group for the random forest algorithm (supervised learning algorithm) and tested by cross validation. For cases with repeated misclassification unsupervized clustering was performed with the aim to identify to the DNA methylation core group within the histological group. This led to the splitting of various WHO entities (e.g. PNET with 4 molecular subtypes, subependymoma splitting into supratentorial, posterior fossa and spinal subtype). For other rare WHO entities we were either so far not able to obtain enough cases to incorporate the group into the classifier or there was no indication of a group by unsupervized clustering (e.g. astroblastoma). This is why our reference set strongly resembles the WHO classification but is not identical.
Name Description Details
Methylation class (myo-)sarcoma with DICER1 mutation The methylation class "(myo-)sarcoma with DICER1 mutation" is based on tumors with the histological diagnosis of malignant tumors not otherwise specified. Median age is 9 years (age range 6 to 76). Molecularly, all cases of this class carry a DICER1 mutation, either somatic or inherited, often together with MAPK pathway alterations. MyoD expression is frequently observed in younger patients. Patients of higher age frequently lack unambiguous myomarker expression. Copy number profiles can range from relatively flat to complex. The name given here is provisional. show
Methylation class Ewing sarcoma The methylation class "Ewing sarcoma" is based on tumors with a histological small blue round cell phenotype. Location is frequently skeletal, but extraskeletal sites may be prevalent; median age is 22 years (age range 3 to 66). Cases of this class consistently carry a gene fusion between TET- and ETS gene family members mostly resulting in a EWSR1-FLI1 fusion gene. A gain of chromosome 8 can be observed in up to 40% of cases. show
Methylation class alveolar soft part sarcoma The methylation class "alveolar soft part sarcoma" is based on tumors with the histological diagnosis of alveolar soft part sarcoma. Location is the soft tissue in all cases; median age is 15 years (age range 3 to 60). Cases of this class consistently carry a ASPSCR1-TFE1 gene fusion. Most cases show a flat profile in copy number analysis. show
Methylation class angiomatoid fibrous histiocytoma The methylation class "angiomatoid fibrous histiocytoma" is based on tumors with the histological diagnosis of angiomatoid fibrous histiocytoma. Location is the soft tissue in all cases; median age is 11 years (age range 6 to 13). Cases of this class frequently carry a EWSR1-CREB1 gene fusion and most cases show a flat profile in copy number analysis. show
Methylation class angiosarcoma The methylation class "angiosarcoma" is based on tumors with the histological diagnosis of angiosarcoma and rarely epithelioid sarcoma. Location is the soft tissue (including skin) and bone; median age is 67 years (age range 50 to 91). The copy number profile shows numerous alterations with a frequent gain of chromosome arm 1q (>60%), gains on 17q (>50%) and losses on 6q (>50%). Angiosarcomas secondary to irradiation often carry a MYC amplification. show
Methylation class atypical fibroxanthoma / pleomorphic dermal sarcoma The methylation class "atypical fibroxanthoma / pleomorphic dermal sarcoma" is based on tumors with the histological diagnosis of atypical fibroxanthoma and pleomorphic dermal sarcoma. These tumors are almost always located in the actinically-damaged skin of the head and neck region; median age is 80 years (age range 60 to 99); striking predominance in males. Copy number alterations typically involve losses of chromosome arm 9p (>70%) and 13q (>90%). show
Methylation class chondroblastoma The methylation class "chondroblastoma" is based on tumors with the histological diagnosis of chondroblastoma. Cases of this class most commonly arise in tubular bones of the limbs; median age is 17 years (age range 14 to 41). Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class chondrosarcoma (IDH wild type group A) The methylation class "chondrosarcoma (IDH wild type group A)" is based on tumors with the histological diagnosis of conventional chondrosarcoma. Location can be any bone; median age is 63 years (age range 33 to 82). Cases of this methylation class are IDH1/2 wild type. Copy number alterations are predominantly whole-chromosome losses and typically involve chromosome arm 1p, 6q, 10q 13q and 14q (each 90%). show
Methylation class chondrosarcoma (IDH wild type group B) The methylation class "chondrosarcoma (IDH wild type group B)" is based on tumors with the histological diagnosis of conventional chondrosarcoma. Location can be any bone; median age is 56 years (age range 30 to 82). Most cases of this methylation class are IDH1/2 wild type (~80%). The copy number analysis shows numerous alterations with a frequent gain of chromosome 19 (>50%) and loss of chromosome 9p, 10p and 13q (>40% each). A homozygeous deletion of the CDKN2A locus on 9p is frequently observed (>50%). show
Methylation class chondrosarcoma (clear cell) The methylation class "chondrosarcoma (clear cell)" is based on tumors with the histological diagnosis of clear cell chondrosarcoma. Location is most commonly a tubular bone of the limbs; median age is 40 years (age range 22 to 49); predominance in males. Copy number analysis frequently shows a gain of chromosome 5 (>50%). show
Methylation class chondrosarcoma (extraskeletal myxoid) The methylation class "chondrosarcoma (extraskeletal myxoid)" is based on tumors with the histological diagnosis of extraskeletal myxoidl chondrosarcoma. Cases of this class most commonly arise in the soft tissue of the limbs; median age is 54 years (age range 42 to 67). A rearrangement of NR4A3, frequenetly with EWSR1, is characteristic for this methylation class. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class chondrosarcoma (mesenchymal) The methylation class "chondrosarcoma (mesenchymal)" is based on tumors with the histological diagnosis of mesenchymal chondrosarcoma. Location can be skeletal and extraskeletal; median age is 19 years (age range 15 to 51). Cases of this class frequently carry a HEY1-NCOA2 gene fusion. The copy number analysis shows sparse alterations with a recurrent gain of chromosome arm 12q (>30%) and loss of chromosome 8p (>30%). show
Methylation class chordoma The methylation class "chordoma" is based on tumors with the histological diagnosis of chordoma. Location is the axial region mostly affecting the skull base; median age is 58 years (age range 14 to 79). The copy number analysis shows numerous alterations with a frequent gain of chromosome arm 1q (60%) and loss of chromosome 1p, 3, 13 and 14 (60% each). All cases are Brachyury positiv. show
Methylation class chordoma (dedifferentiated) The methylation class "chordoma (dedifferentiated)" is based on tumors with the histological diagnosis of poorly differentiated chordoma. Location is mostly the skull base; median age is 7 years (age range 1 to 17). Copy number analysis shows a recurrent deletion of the SMARCB1 locus on chromosome arm 22q. Otherwise, the copy number profile is flat. All cases of this class are Brachyury positive and INI-1 negative. show
Methylation class clear cell sarcoma (kidney) The methylation class "clear cell sarcoma (kidney)" is based on tumors with the histological diagnosis of clear cell sarcoma of the kidney. Median age is xx years (age range xx to xx). The BCOR internal tandem duplication is characteristic for this methylation class. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class control (muscle tissue) The methylation class " control (muscle tissue)" is comprised of normal tissue samples from skeletal muscle. In case tumor samples display this molecular pattern, it is typically an indication of low tumor cell content in the analysed material and indicates that the extracted DNA is likely not suitable for classification by methylation profiling. show
Methylation class control (reactive tissue) The methylation class "control (reactive tissue)" is comprised of granulation tissue samples originated from vertebral disc prolapses. In case tumor samples display this molecular pattern, it is typically an indication of low tumor cell content in the analysed material and indicates that the extracted DNA is likely not suitable for classification by methylation profiling. show
Methylation class dedifferentiated liposarcoma The methylation class "dedifferentiated liposarcoma" is based on tumors with the histological diagnosis of dedifferentiated liposarcoma. Location is most commonly the deep soft tissue of the trunk; median age is 73 years (age range 45 to 86). Cases of this methylation class are characterized by an amplification on chromosome arm 12q13-15 involving the MDM2 locus. Otherwise, the copy number profile shows numerous chromosomal alterations. show
Methylation class dermatofibrosarcoma protuberans The methylation class "dermatofibrosarcoma protuberans" is based on tumors with the histological diagnosis of dermatofibrosarcoma protuberans. Location is the skin and subcutaneous soft tissue of the trunk and limbs; median age is 33 years (age range 2 to 72). The COL1A1-PDGFB fusion gene based on a supernumerary ring chromosome comprising sequences of chromosomes 17q and 22q is characteristic for cases of this class. Accordingly, gains on chromosome arms 17q and 22q are frequently observed in copy number analysis. show
Methylation class desmoid-type fibromatosis The methylation class "desmoid-type fibromatosis" is based on tumors with the histological diagnosis of desmoid-type fibromatosis. Location is frequently the trunk; median age is 50 years (age range 30 to 71). Cases of this class often present with an abnormal nuclear accumulation of ß-catenin. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class desmoplastic small round cell tumor The methylation class "desmoplastic small round cell tumor" is based on tumors with the histological diagnosis of desmoplastic small round cell tumor. Location is frequently the trunk; median age is 17 years (age range 6 to 46); striking predominance in males. Cases of this class consistently carry an EWSR1-WT1 gene fusion. A gain of chromosome arm 1q, 3, 5 and/or 21q can be observed in up to 50% of cases. show
Methylation class endometrial stromal sarcoma (low grade) The methylation class "endometrial stromal sarcoma (low-grade)" is based on tumors with the histological diagnosis of low-grade endometrial stromal sarcoma. Location is frequently the uterus, if not associated with an extrauterine endometriosis; median age is xx years (age range xx to xx). Cases of this class frequently carry rearrangements involving JAZF1, SUZ12 or PHF1. Recurrent choromosomal alterations involve a loss of chromosome arm 7p (>50%). High-grade tumors are often associated with rearragements involving YWHAE, FAM22 or BCOR. They do not assign to this class. show
Methylation class epithelioid hemangioendothelioma The methylation class "epithelioid hemangioendothelioma" is based on tumors with the histological diagnosis of epithelioid hemangioendothelioma. Location is the superficial and deep soft tissue, but epithelioid hemangioendothelioma may also arise in the skeleton; median age is 48 years (age range 10 to 70). Cases of this class characteristically carry a WWTR1-CAMTA1 gene fusion or less frequently a YAP1-TFE3 gene fusion. Many cases show a flat profile in copy number analysis. show
Methylation class epithelioid sarcoma The methylation class "epithelioid sarcoma" is based on tumors with the histological diagnosis of epithelioid sarcoma, either classic or proximal-type. Location is frequently the extremity; median age is 37 years (age range 19 to 68). Copy number analysis shows a high frequency of SMARCB1 deletions on chromosome arm 22q (> 70%) and losses on 8p (50%). Almost all cases of this methylation class present with a complete loss of nuclear INI-1 expression. show
Methylation class fibrous dysplasia The methylation class "fibrous dysplasia" is based on tumors with the histological diagnosis of fibrous dysplasia, either in its monostotic or polyostotic form. Location is the skeleton, frequently a bone of the extremities; median age is 34 years (age range 15 to 61). Cases of this class consistenly carry a GNAS hotspot mutation at codon 201. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class gastrointestinal stromal tumor The methylation class "gastrointestinal stromal tumor" is based on tumors with the histological diagnosis of gastrointestinal stromal tumor. Location is mostly the gastrointestinal tract; median age is 63 years (age range 30 to 94). Cases of this class frequently carry a KIT or PDGFRA mutation. Recurrent chromosomal alterations involve a loss of chromosome 14 (70%), 1p and 22q (each 40%). show
Methylation class giant cell tumor of bone The methylation class "giant cell tumor of bone" is based on tumors with the histological diagnosis of giant cell tumor of bone. Cases mostly arise in the epiphyseal-metaphyseal region of long tubular bones; median age is 29 years (age range 22 to 75). Cases of this class consistenly carry a H3F3A G34W/L hotspot mutation. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class granular cell tumor The methylation class "granular cell tumor" is based on tumors with the histological diagnosis of granular cell tumor. Cases mostly arise in subcutaneous/submucosal location; median age is 44 years (age range 26 to 65); slight female predilection. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class infantile fibrosarcoma The methylation class "infantile fibrosarcoma" is based on tumors with the histological diagnosis of infantile fibrosarcoma. Location is the superficial and deep soft tissue of the extremities, trunk and head/neck; median age is <1 years (age range 0 to 1). The ETV6-NTRK3 fusion gene is characteristic for cases of this methylation class. Recurrent chromosomal alterations involve a gain of chromosome 11 (70%), 17 and 20 (each 50%). show
Methylation class inflammatory myofibroblastic tumor The methylation class "inflammatory myofibroblastic tumor" is based on tumors with the histological diagnosis of inflammatory myofibroblastic tumor. These tumors are located in the soft tissue and viscera; median age is 10 years (age range 2 to 17). ALK rearrangements are frequently found in cases of this class. Most cases show a flat profile in copy number analysis. show
Methylation class leiomoysarcoma The methylation class "leiomoysarcoma" is based on tumors with the histological diagnosis of leiomoysarcoma or a small proportion of high-grade sarcomas not otherwise specified. These tumors are mostly located in the retroperitoneum; median age is 57 years (age range 39 to 87). Copy number alterations are numerous with a frequent loss of chromosomes 13 (>80%), 10 (>60%) and 16q (>60%). Gains frequently involve chromosome arm 5p, 8q and 17p (40% each). show
Methylation class lipoma The methylation class "lipoma" is based on tumors with the histological diagnosis of lipoma. These tumors are mostly located in the superficial soft tissue; median age is 58 years (age range 29 to 79). Recurrent chromosomal alterations, especially MDM2 amplifications, are not observed and most cases show a flat profile in copy number analysis. show
Methylation class low-grade fibromyxoid sarcoma The methylation class "low-grade fibromyxoid sarcoma" is based on tumors with the histological diagnosis of low-grade fibromyxoid sarcoma. These tumours are most common in proximal extremities and the trunk; median age is 34 years (age range 18 to 56). Cases of this class consistently carry a FUS-CREB3L2/1 gene fusion. Most cases show a flat profile in copy number analysis. show
Methylation class malignant peripheral nerve sheath tumor The methylation class "malignant peripheral nerve sheath tumor" is based on tumors with the histological diagnosis of malignant peripheral nerve sheath tumor, either sporadic or associated with Von Recklinghausen's Disease (neurofibromatosis type 1). Cases of this class are mostly located in the retroperitoneum and the extremities; median age is 22 years (age range 9 to 54). Copy number alterations are numerous with frequent gains involving chromosome arm 7p (>50%), 8q (>80%), segmental gains of 17q (>70%), and losses of chromosome arm 9p (>70%), 13 (>80%), 10 (>60%), 16q (>60%) and a distinct segmental deletion of 17q11.2 (>60%) covering the NF1 locus. All cases of this class have a loss of histone H3K27 trimethylation. show
Methylation class malignant rhabdoid tumor The methylation class "malignant rhabdoid tumor" is based on tumors with the histological diagnosis of malignant rhabdoid tumor. Cases of this class comprises tumors located in the renal and extrarenal region (without the CNS); median age is 1 year (age range 0 to 14). A segmental deletion chromosome arm 22q11.2 involving the SMARCB1 locus can be observed in up to 80% of cases. Almost all cases of this methylation class present with a complete loss of nuclear INI-1 expression. show
Methylation class melanotic schwannoma The methylation class "melanotic schwannoma" is based on tumors with the histological diagnosis melanotic schwannoma. Location is typically along the spinal meninges and frequently cervical; median age is 38 years (range 11 to 64). Numeric whole chromosome changes are frequent in this class, with gain of chromosome 5, 7 and 9 (> 50% of cases) and loss of chromosome 21 and 22 (>30%) being most frequent. Molecularly, theses tumors frequently carry mutations in PRKAR1A. show
Methylation class myositis ossificans The methylation class "myositis ossificans" is based on tumors with the histological diagnosis myositis ossificans. They arise often in locations susceptible to trauma such as extremities, shoulder, buttock; median age is 13 years (range 9 to 17). Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class myositis proliferans The methylation class "myositis proliferans" is based on tumors with the histological diagnosis myositis proliferans. By definition, they arise in intamuscular location. The subcutaneous counterpart has the same cellular composition and is termed proliferative fasciitis. The median age is 64 years (range 28 to 79). Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class myxoid liposarcoma The methylation class "myxoid liposarcoma" is based on tumors with the histological diagnosis myxoid liposarcoma. Location is typically the deep soft tissue of extremities, mainly within the muscles of the thigh. The median age is 52 years (range 32 to 84). Molecularly, these cases are characterized by a FUS-DDIT3 fusion gene (>95%) or by a EWSR1-DDIT3 fusion gene (<5%). Myxoid liposarcomas frequently carry a TERT promoter mutation (>70%). Most cases show a flat profile in copy number analysis. show
Methylation class neurofibroma The methylation class "neurofibroma" is based on tumors with the histological diagnosis of neurofibroma, either sporadic or associated with Von Recklinghausen's Disease (neurofibromatosis type 1). Cases of this class are most commonly located in the skin; median age is 32 years (age range 17 to 43). The copy number profile is flat except for a distinct segmental deletion of 17q11.2 covering the NF1 locus in up to 40% of cases. show
Methylation class neurofibroma (plexiform) The methylation class "plexiform neurofibroma" is based on tumors with the histological diagnosis of plexiform neurofibroma of the plexiform variant, either sporadic or associated with Von Recklinghausen's Disease (neurofibromatosis type 1). Plexiform neurofibromas have a slightly increased risk for malignant transformation. Cases of this class are most commonly located in the skin; median age is 13 years (age range 5 to 39). The copy number profile is flat except for a distinct segmental deletion of 17q11.2 covering the NF1 locus in up to 30% of cases. show
Methylation class nodular fasciitis The methylation class "nodular fasciitis" is based on tumors with the histological diagnosis of nodular fasciitis. Cases of this class are most commonly located in the subcutaneous soft tissue; median age is 13 years (age range 5 to 39). Cases of this class frequently carry a MYH9-USP6 gene fusion. Recurrent chromosomal alterations are not observed. Most cases show a flat profile in copy number analysis. show
Methylation class ossifying fibromyxoid tumor The methylation class "ossifying fibromyxoid tumor" is based on tumors with the histological diagnosis of ossifying fibromyxoid tumor. Cases of this class are most commonly located in the soft tissue of extremitites and the trunk; median age is 50 years (age range 41 to 77). Cases of this class frequently carry rearrangements of chromosome arm 6p21 involving PHF1. In copy number analysis up to 40% of cases show a loss of chromosome arm 6q. show
Methylation class osteoblastoma The methylation class "osteoblastoma" is based on tumors with the histological diagnosis of osteoblastoma. Cases of this class are most commonly located in tubular bones, the spine and the craniofascial bones (mandible); median age is 15 years (age range 8 to 28). Characteristic molecular features of this class are not known. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylation class osteosarcoma (conventional) The methylation class "osteosarcoma (conventional)" is based on tumors with the histological diagnosis of conventional osteosarcoma. These tumors are mostly located in the tubular bones of the extremities; median age is 15 years (age range 5 to 78). Copy number alterations are numerous with frequent losses on chromosome 5q (>50%), 6q (>60%) 10 (>60%), 13q (>60%), and gains on chromosome arm 1p, 6p (each >40%), 8q and 17p (each >60%). show
Methylation class rhabdomyosarcoma (alveolar) The methylation class "rhabdomyosarcoma (alveolar)" is based on tumors with the histological diagnosis of alveolar rhabdomyosarcoma. Primary location is frequently the deep soft tissue of extremities, this class is predominantly composed of metastasic cases; median age is 13 years (age range 2 to 26). Cases of this class frequently carry a FOXO1 (FKHR) rearrangement. The copy number profile frequently shows numerous copy number alterations. Amplifications are frequent on chromosome arm 12q13-15 involving the CDK4 locus (~20%) and chromosome arm 2p24 involving the MYCN locus (~20%). show
Methylation class rhabdomyosarcoma (embryonal) The methylation class "rhabdomyosarcoma (embryonal)" is based on tumors with the histological diagnosis of embryonal rhabdomyosarcoma, including the botryoid variant. Location is frequently the head and neck region or the genitourinary system; median age is 12 years (age range 1 to 30). Copy number alterations are numerous with frequent whole-chromosome gains of chromosome 8 (>80%), 2 (>60%), 11, 12, 13 and 20 (>40%). Copy number analysis shows a high frequency of CDKN2A/B deletions on chromosome arm 9p (> 40%). show
Methylation class sarcoma (paraspinal, H3K27me3 retained) The methylation class "sarcoma (paraspinal, H3K27me3 retained)" is based on tumors with morphological features typical of malignant peripheral nerve sheath tumor, however, with retained expression of the H3K27 trimethylation marker. These tumors are located (para-)spinal; median age is 50 years (age range 10 to 77). Copy number alterations are numerous with a frequent loss of chromosome arm 3q (>80%), 5q (>80%), 9p (100%) and 17q (>70%). Copy number analysis shows a high frequency of CDKN2A/B homozygeous deletions on chromosome arm 9p (> 40%). Further molecular features of this class include mutations in the NF1 gene. The name given here is provisional. show
Methylation class schwannoma The methylation class "schwannoma" is based on tumors with the histological diagnosis of schwannoma. Location is frequently a peripheral nerve of the upper extremity followed by cranial nerve VIII; median age is 26 years (age range 12 to 64). The copy number profile is flat except for a deletion of chromosome arm 22q covering the NF2 locus in up to 40% of cases. show
Methylation class small blue round cell tumor with BCOR alteration The methylation class "small blue round cell tumor with BCOR alteration" is based on tumors with the histological diagnosis of undifferentiated small blue round cell tumor, not otherwise specified, Ewing sarcoma and Ewing-like sarcoma. Tumors most commony carry BCOR-CCNB3 gene fusions, arise in soft tissue or bone of children, adolecents or young adults (age range to 20 years). Tumors in infants (<1 year) typically carry BCOR internal tandem duplications or point mutations. Numeric copy number alterations are sparse and most cases of this class show a flat profile in copy number analysis. CNS high-grade neuroepithelial tumor with BCOR alteration (see brain tumor classifier) is the CNS counterpart for this class. show
Methylation class small blue round cell tumor with CIC alteration The methylation class "small blue round cell tumor with CIC alteration" is based on tumors with the histological diagnosis of Ewing sarcoma, Ewing-like sarcoma and undifferentiated small blue round cell sarcoma, not otherwise specified. Location is almost always the soft tissue; median age is 38 years (age range 16 to 49). Cases of this class frequently carry CIC-DUX2/4 gene fusions. Overall, copy number alterations are sparse. However, up to 40% of cases show a segmental loss on chromosome arm 1p. CNS high-grade neuroepithelial tumor with CIC alteration (see brain tumor classifier) is the CNS counterpart for this class. show
Methylatoin class chondrosarcoma (IDH mutant group A) The methylation class "chondrosarcoma (IDH mutant group A)" is based on tumors with the histological diagnosis of conventional chondrosarcoma. Location is most frequently the skull base; median age is 44 years (age range 17 to 73). Cases of this methylation class carry an IDH1/2 mutations. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylatoin class chondrosarcoma (IDH mutant group B) The methylation class "chondrosarcoma (IDH mutant group A)" is based on tumors with the histological diagnosis of conventional chondrosarcoma. Location is predominantly the limbs; median age is 48 years (age range 16 to 79). Most cases of this methylation class carry IDH1/2 mutations. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylatoin class solitary fibrous tumor The methylation class "solitary fibrous tumor" is based on tumors with the histological diagnosis of solitary fibrous tumor. Location is the intracranial and peripheral soft tissue; median age is 59 years (age range 15 to 83). Cases of this class consistently carry a NAB2-STAT6 gene fusion. Cases of this class consistently show a strong nuclear relocation in the STAT6 staining. Recurrent chromosomal alterations are not observed and most cases show a flat profile in copy number analysis. show
Methylatoin class squamous cell carcinoma (cutaneous) The methylation class "squamous cell carcinoma (cutaneous)" is based on tumours with the histological diagnosis of cutaneous squamous cell carcinoma. Location is mostly the actinically-damaged skin of the head and neck region; median age is 74 years (age range 55 to 90). Molecularly, cases of this class frequently carry TERT promoter mutations. Copy number alterations are numerous with frequent losses on chromosome arm 9p (>60%), 18q (>40%) and gains on chromosome arm 3q and 8q (each >30%). show
Methylatoin class synovial sarcoma The methylation class "synovial sarcoma" is based on tumors with the histological diagnosis of synovial sarcoma. Location is mostly the soft tissue of extremities; median age is 37 years (age range 13 to 69). Cases of this class consistently carry a SS18-SSX gene fusion. Most cases show a flat profile in copy number analysis. show
Methylatoin class undifferentiated pleomorphic sarcoma The methylation class "undifferentiated pleomorphic sarcoma" is based on tumors with the histological diagnosis of undifferentiated pleomorphic sarcoma. Location is the soft tissue mostly of the extremitites; median age is 73 years (age range 38 to 85). The copy number analysis shows numerous alterations with frequent gains on chromosome arm 1p and 7p (each (60%) and losses on chromosome 1q (80%), 2q (70%), 9p (>50%), 10 (>60%), 11q (>50%), 11q (>50%), 12p (>50%), 13q (>60%) and 17p (>50%). show